Identification and Characterization ofgshA, a Gene Encoding the Glutamate-Cysteine Ligase in the Halophilic ArchaeonHaloferax volcanii
Copyright policy )Identification and Characterization ofgshA, a Gene Encoding the Glutamate-Cysteine Ligase in the Halophilic ArchaeonHaloferax volcanii
ABSTRACTHalophilic archaea were found to contain in their cytoplasm millimolar concentrations of γ-glutamylcysteine (γGC) instead of glutathione. Previous analysis of the genome sequence of the archaeonHalobacteriumsp. strain NRC-1 has indicated the presence of a sequence homologous to sequences known to encode the glutamate-cysteine ligase GshA. We report here the identification of thegshAgene in the extremely halophilic archaeonHaloferax volcaniiand show thatH. volcanii gshAdirects in vivo the synthesis and accumulation of γGC. We also show that theH. volcaniigene when expressed in anEscherichia colistrain lacking functional GshA is able to restore synthesis of glutathione.
- Tel Aviv University Israel
Sequence Homology, Amino Acid, Archaeal Proteins, Glutamate-Cysteine Ligase, Genetic Complementation Test, Molecular Sequence Data, Dipeptides, Amino Acid Sequence, Gene Expression Regulation, Archaeal, Haloferax volcanii, Chromatography, High Pressure Liquid, Phylogeny
Sequence Homology, Amino Acid, Archaeal Proteins, Glutamate-Cysteine Ligase, Genetic Complementation Test, Molecular Sequence Data, Dipeptides, Amino Acid Sequence, Gene Expression Regulation, Archaeal, Haloferax volcanii, Chromatography, High Pressure Liquid, Phylogeny
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).20 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!