An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity
Copyright policy )An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity
Significance Regulation of stem cells by microenvironment signals is important to maintain epithelial homeostasis. Using a quantitative readout, we screened for receptor genes that affect the intestinal stem cell pool size in the adult Drosophila midgut. The top candidate of our screen, AdoR , underscores the importance of purinergic signaling in controlling ISC activity. Furthermore, we identified a pivotal role of an enterocyte-derived metabolic enzyme, Adgf-A, in limiting the activity of extracellular adenosine and shaping the ISC microenvironment.
- Howard Hughes Medical Institute United States
- Harvard University United States
- Boston Children's Hospital United States
Adenosine, Adenosine Deaminase, MAP Kinase Signaling System, Multipotent Stem Cells, Receptors, Purinergic P1, Down-Regulation, Cell Differentiation, Animals, Genetically Modified, Enterocytes, Gene Knockdown Techniques, Animals, Drosophila Proteins, Drosophila, RNA Interference, Gene Knock-In Techniques, Cell Proliferation
Adenosine, Adenosine Deaminase, MAP Kinase Signaling System, Multipotent Stem Cells, Receptors, Purinergic P1, Down-Regulation, Cell Differentiation, Animals, Genetically Modified, Enterocytes, Gene Knockdown Techniques, Animals, Drosophila Proteins, Drosophila, RNA Interference, Gene Knock-In Techniques, Cell Proliferation
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