RNA Interference of Interferon Regulatory Factor-1 Gene Expression in THP-1 Cell Line Leads to Toll-Like Receptor-4 Overexpression/Activation As Well As Up-modulation of Annexin-II
Copyright policy )RNA Interference of Interferon Regulatory Factor-1 Gene Expression in THP-1 Cell Line Leads to Toll-Like Receptor-4 Overexpression/Activation As Well As Up-modulation of Annexin-II
Interferon regulatory factor-1 (IRF-1) is a candidate transcription factor for the regulation of the Toll-like receptor-4 (TLR-4) gene. Using a small interfering RNA-based (siRNA) process to silence IRF-1 gene expression in the leukemic monocytic cell line THP-1, we investigated whether such a modulation would alter TLR-4 expression and activation status in these cells. The siIRF-1 cells expressed elevated levels of TLR-4 mRNA and protein compared to controls by 90% and 77%, respectively. ICAM.1 protein expression and apoptosis levels were increased by 8.35- and 4.25-fold, respectively. The siIRF-1 cells overexpressed Bax mRNA compared to controls. Proteomic analysis revealed upmodulation of the Annexin-II protein in siIRF-1 THP-1 cells. Myelodysplastic syndrome (MDS) patients with an absence of full-length IRF-1 mRNA also overexpressed Annexin-II. It is plausible that this overexpression may lead to the activation of TLR-4 contributing to the increased apoptosis characterizing MDS.
Molecular Sequence Data, Apoptosis, Bone Marrow Cells, Transfection, Monocytes, Annexin II, Leukemia, Promyelocytic, Acute, Humans, Amino Acid Sequence, RNA, Messenger, RNA, Small Interfering, RC254-282, Annexin A2, Cells, Cultured, bcl-2-Associated X Protein, IRF-1, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, TLR-4, Intercellular Adhesion Molecule-1, myelodysplastic syndrome, Protein Structure, Tertiary, Toll-Like Receptor 4, Gene Expression Regulation, RNAi, Myelodysplastic Syndromes, RNA Interference, Interferon Regulatory Factor-1
Molecular Sequence Data, Apoptosis, Bone Marrow Cells, Transfection, Monocytes, Annexin II, Leukemia, Promyelocytic, Acute, Humans, Amino Acid Sequence, RNA, Messenger, RNA, Small Interfering, RC254-282, Annexin A2, Cells, Cultured, bcl-2-Associated X Protein, IRF-1, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, TLR-4, Intercellular Adhesion Molecule-1, myelodysplastic syndrome, Protein Structure, Tertiary, Toll-Like Receptor 4, Gene Expression Regulation, RNAi, Myelodysplastic Syndromes, RNA Interference, Interferon Regulatory Factor-1
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