Sonic Hedgehog Is Required for Progenitor Cell Maintenance in Telencephalic Stem Cell Niches
Copyright policy )Sonic Hedgehog Is Required for Progenitor Cell Maintenance in Telencephalic Stem Cell Niches
To directly test the requirement for hedgehog signaling in the telencephalon from early neurogenesis, we examined conditional null alleles of both the Sonic hedgehog and Smoothened genes. While the removal of Shh signaling in these animals resulted in only minor patterning abnormalities, the number of neural progenitors in both the postnatal subventricular zone and hippocampus was dramatically reduced. In the subventricular zone, this was partially attributable to a marked increase in programmed cell death. Consistent with Hedgehog signaling being required for the maintenance of stem cell niches in the adult brain, progenitors from the subventricular zone of floxed Smo animals formed significantly fewer neurospheres. The loss of hedgehog signaling also resulted in abnormalities in the dentate gyrus and olfactory bulb. Furthermore, stimulation of the hedgehog pathway in the mature brain resulted in elevated proliferation in telencephalic progenitors. These results suggest that hedgehog signaling is required to maintain progenitor cells in the postnatal telencephalon.
- University of Queensland Australia
- New York University Langone Medical Center United States
- New York University United States
- University of Gothenburg Sweden
- University of Queensland Australia
Male, Mice, Knockout, Telencephalon, Cell Death, Mammalian Telencephalon, Neuroscience(all), Stem Cells, In-Situ Hybridization, Gene Expression Regulation, Developmental, Central-Nervous-System, Mice, Inbred C57BL, Subventricular Zone Cells, Mice, Trans-Activators, Animals, Oligodendrocyte Development, Female, Hedgehog Proteins, Cells, Cultured
Male, Mice, Knockout, Telencephalon, Cell Death, Mammalian Telencephalon, Neuroscience(all), Stem Cells, In-Situ Hybridization, Gene Expression Regulation, Developmental, Central-Nervous-System, Mice, Inbred C57BL, Subventricular Zone Cells, Mice, Trans-Activators, Animals, Oligodendrocyte Development, Female, Hedgehog Proteins, Cells, Cultured
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).608 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!