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Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster

Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster
We constructed Drosophila melanogaster bacterial artificial chromosome libraries with 21-kilobase and 83-kilobase inserts in the P[acman] system. We mapped clones representing 12-fold coverage and encompassing more than 95% of annotated genes onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using UC31 integrase to rescue mutations. They can be modified through recombineering, for example, to incorporate protein tags and assess expression patterns.
- University of North Texas United States
- Howard Hughes Medical Institute United States
- Baylor College of Medicine United States
- Lawrence Berkeley National Laboratory United States
- Children's Hospital Oakland Research Institute United States
570, Chromosomes, Artificial, Bacterial, Base Sequence, Molecular Sequence Data, 59, Proteins, 610, Chromosome Mapping, Article, Animals, Genetically Modified, Drosophila melanogaster, Genes, Animals, Drosophila, Cloning, Molecular, Mutations, Gene Library
570, Chromosomes, Artificial, Bacterial, Base Sequence, Molecular Sequence Data, 59, Proteins, 610, Chromosome Mapping, Article, Animals, Genetically Modified, Drosophila melanogaster, Genes, Animals, Drosophila, Cloning, Molecular, Mutations, Gene Library
201 Research products, page 1 of 21
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