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Nature Methods
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Other literature type . 2009
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Nature Methods
Article . 2009 . Peer-reviewed
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Nature Methods
Article . 2009
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Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster

Authors: Venken, Koen J.T.; Carlson, Joseph W.; Schulze, Karen L.; Pan, Hongling; He, Yuchun; Spokony, Rebecca; Wan, Kenneth H.; +5 Authors

Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster

Abstract

We constructed Drosophila melanogaster bacterial artificial chromosome libraries with 21-kilobase and 83-kilobase inserts in the P[acman] system. We mapped clones representing 12-fold coverage and encompassing more than 95% of annotated genes onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using UC31 integrase to rescue mutations. They can be modified through recombineering, for example, to incorporate protein tags and assess expression patterns.

Country
United States
Keywords

570, Chromosomes, Artificial, Bacterial, Base Sequence, Molecular Sequence Data, 59, Proteins, 610, Chromosome Mapping, Article, Animals, Genetically Modified, Drosophila melanogaster, Genes, Animals, Drosophila, Cloning, Molecular, Mutations, Gene Library

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    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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    influence
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    Top 1%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
365
Top 1%
Top 1%
Top 1%
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