Loss of promoter methylation contributes to the expression of functionally impaired PRDM1β isoform in diffuse large B-cell lymphoma
Copyright policy )Loss of promoter methylation contributes to the expression of functionally impaired PRDM1β isoform in diffuse large B-cell lymphoma
The positive regulatory domain I (PRDM1) is a transcriptional repressor that plays an important role in the B-cell differentiation. PRDM1β isoform is functionally impaired and overexpressed in a subset of diffuse large B-cell lymphoma with aggressive behavior. To investigate the possible epigenetic alteration on PRDM1β expression, methylation of PRDM1β promoter was assessed in B-lymphoma cell lines by bisulfite-sequencing PCR and screened in tumor samples of DLBCL patients using MassARRAY-quantitative methylation analysis. PRDM1β expression corresponded to promoter methylation status. CpG island of PRDM1β promoter possessed significant transcriptional activity, which could be modified by methylation. Loss of promoter methylation of PRDM1β was more frequently detected in lymphoma samples than in reactive hyperplasia. Thus, demethylation of abnormal tumor suppressor gene isoform might be linked to lymphoma progression.
- Shanghai Institute of Hematology China (People's Republic of)
- State Key Laboratory of Medical Genomics China (People's Republic of)
- Shanghai Jiao Tong University China (People's Republic of)
- Shanghai University China (People's Republic of)
Epigenomics, Repressor Proteins, Cell Line, Tumor, Humans, Protein Isoforms, CpG Islands, Lymphoma, Large B-Cell, Diffuse, Positive Regulatory Domain I-Binding Factor 1, DNA Methylation, Promoter Regions, Genetic
Epigenomics, Repressor Proteins, Cell Line, Tumor, Humans, Protein Isoforms, CpG Islands, Lymphoma, Large B-Cell, Diffuse, Positive Regulatory Domain I-Binding Factor 1, DNA Methylation, Promoter Regions, Genetic
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