Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kdcomplexed with an HBV-core nonapeptide
Copyright policy )Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kdcomplexed with an HBV-core nonapeptide
In order to establish a system for structural studies of the murine class I major histocompatibility antigen complex (MHC) H-2Kd, a bacterial expression system and in vitro refolding preparation of the complex of H-2Kd with human beta2m and the immunodominant peptide SYVNTNMGL from hepatitis B virus (HBV) core-protein residues 87-95 was employed. The complex (45 kDa) was crystallized; the crystals belong to space group P222(1), with unit-cell parameters a = 89.082, b = 110.398, c = 47.015 A, alpha = beta = gamma = 90 degrees. The crystals contain one complex per asymmetric unit and diffract X-rays to at least 2.06 A resolution. The structure has been solved by molecular replacement and is the first crystal structure of a peptide-H-2Kd complex.
- Tsinghua University China (People's Republic of)
- Northamptonshire Healthcare NHS Foundation Trust United Kingdom
- Oxford University Hospitals NHS Trust United Kingdom
- Chinese Academy of Sciences China (People's Republic of)
- John Radcliffe Hospital United Kingdom
Hepatitis B virus, Protein Folding, Viral Core Proteins, H-2 Antigens, Chromatography, Ion Exchange, Crystallography, X-Ray, Peptide Fragments, Mice, Chromatography, Gel, Animals, Humans, Amino Acid Sequence, Crystallization, beta 2-Microglobulin, Protein Binding
Hepatitis B virus, Protein Folding, Viral Core Proteins, H-2 Antigens, Chromatography, Ion Exchange, Crystallography, X-Ray, Peptide Fragments, Mice, Chromatography, Gel, Animals, Humans, Amino Acid Sequence, Crystallization, beta 2-Microglobulin, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).25 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!