Altered Molecular Regionalization and Normal Thalamocortical Connections in Cortex-SpecificPax6Knock-Out Mice
Copyright policy )Altered Molecular Regionalization and Normal Thalamocortical Connections in Cortex-SpecificPax6Knock-Out Mice
Transcription factor Pax6 exerts a prominent rostrolateralhighto caudomediallowexpression gradient in the cortical progenitors and have been implicated in regulation of area identity in the mammalian cortex. Herein, we analyzed the role of Pax6 in molecular arealization and development of thalamocortical connections in the juvenile cortex-specific conditionalPax6knock-out mice (Pax6cKO). Using a set of molecular markers of positional identity (Id2,Cadherin6,COUP-TF1,RZRβ, andEphA7), we show that, in the juvenilePax6cKO, the relative size of caudal cortical areas (putative visual and somatosensory) are mildly enlarged, whereas the rostral domain (putative motor) is severely reduced. Despite the rostral shift of graded expression of areal markers, the distribution of area-specific thalamocortical and corticofugal projections appear normal in thePax6cKO. This indicates that change of the size of cortical areas is not accompanied by a change in cortical identity. We show furthermore that, despite a severe depletion of supragranular cortical layers and accumulation of cells along the pallial–subpallial boundary, thalamocortical fibers establish a periphery-related pattern of the somatosensory cortex in normal position inPax6cKO.Our findings indicate that Pax6 expression gradients in cortical progenitors do not directly impart thalamocortical or corticofugal areal identity.
- University of Oxford United Kingdom
- Max Planck Society Germany
Cerebral Cortex, Homeodomain Proteins, COUP Transcription Factor I, PAX6 Transcription Factor, Gene Expression Regulation, Developmental, Nuclear Receptor Subfamily 1, Group F, Member 2, Receptors, Cytoplasmic and Nuclear, Mice, Transgenic, Receptor, EphA7, Cadherins, Mice, Inbred C57BL, Repressor Proteins, Mice, Animals, Newborn, Neural Pathways, Morphogenesis, Animals, Paired Box Transcription Factors, Eye Proteins, Inhibitor of Differentiation Protein 2
Cerebral Cortex, Homeodomain Proteins, COUP Transcription Factor I, PAX6 Transcription Factor, Gene Expression Regulation, Developmental, Nuclear Receptor Subfamily 1, Group F, Member 2, Receptors, Cytoplasmic and Nuclear, Mice, Transgenic, Receptor, EphA7, Cadherins, Mice, Inbred C57BL, Repressor Proteins, Mice, Animals, Newborn, Neural Pathways, Morphogenesis, Animals, Paired Box Transcription Factors, Eye Proteins, Inhibitor of Differentiation Protein 2
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