Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family
Copyright policy )Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family
The Zic-family proteins control various developmental processes. Previous studies have shown that Zic1, Zic2, and Zic3 can act as transcriptional regulators, and that their functions are repressed by I-mfa, which has been identified as a repressor for basic helix-loop-helix-type transcriptional factors. Here, we investigated the molecular properties of the Zic4 and Zic5 proteins. Zic4/Zic5 showed DNA-binding activity to the Gli-binding sequence, similar to Zic1/Zic2/Zic3 proteins. However, Zic4/Zic5 did not exhibit any significant transcriptional activation ability nor they bind to I-mfa differently from Zic1/Zic2/Zic3. The nuclear localization of Zic4/Zic5 was not affected by the presence of the I-mfa protein, whereas the Zic1/Zic2/Zic3 proteins were translocated to the cytoplasmic compartment in the presence of I-mfa. The difference may be attributable to the dissimilarity of the N-terminal region between the Zic1/Zic2/Zic3 and Zic4/Zic5 proteins, since the binding of the Zic1/Zic2/Zic3 proteins to I-mfa occurs through their N-terminal regions.
Homeodomain Proteins, Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Zinc Fingers, DNA, Cell Line, DNA-Binding Proteins, Repressor Proteins, Mice, Gene Expression Regulation, Myogenic Regulatory Factors, NIH 3T3 Cells, Animals, Humans, Amino Acid Sequence, Carrier Proteins, Protein Binding, Transcription Factors
Homeodomain Proteins, Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Zinc Fingers, DNA, Cell Line, DNA-Binding Proteins, Repressor Proteins, Mice, Gene Expression Regulation, Myogenic Regulatory Factors, NIH 3T3 Cells, Animals, Humans, Amino Acid Sequence, Carrier Proteins, Protein Binding, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).25 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!