Structure and expression of an asparaginyl-tRNA synthetase gene located on chromosome IV of Arabidopsis thaliana and adjacent to a novel gene of 15 exons
Copyright policy )Structure and expression of an asparaginyl-tRNA synthetase gene located on chromosome IV of Arabidopsis thaliana and adjacent to a novel gene of 15 exons
The gene AtNS1 coding for an asparaginyl-tRNA synthetase and located on chromosome IV of Arabidopsis thaliana has been characterized. AtNS1 is the first asparaginyl-tRNA synthetase gene described in higher plants. The genomic environment of AtNS1 has been studied, as well as a partial cDNA of a second homologous asparaginyl-tRNA synthetase gene, AtNS2. Both AtNS1 and AtNS2 exhibit the highest similarity with prokaryotic homologues. A large novel gene of 15 exons, named AtG2484-1, is located adjacent to AtNS1. AtG2484-1 shows features rarely described in plants including large exons and one 3' non-coding exon. PCR and Northern analyses were carried out to obtain information about the expression of these genes in various A. thaliana tissues.
- Département Sciences sociales, agriculture et alimentation, espace et environnement France
- French National Centre for Scientific Research France
- University of Paris-Saclay France
- National Research Institute for Agriculture, Food and Environment France
- Développement Adaptation et Vieillissement France
Base Sequence, DNA, Plant, Sequence Homology, Amino Acid, [SDV]Life Sciences [q-bio], Aspartate-tRNA Ligase, Molecular Sequence Data, Arabidopsis, Chromosome Mapping, Exons, RNA, Transfer, Amino Acyl, Genes, Plant, [SDV] Life Sciences [q-bio], Amino Acyl-tRNA Synthetases, Humans, Amino Acid Sequence
Base Sequence, DNA, Plant, Sequence Homology, Amino Acid, [SDV]Life Sciences [q-bio], Aspartate-tRNA Ligase, Molecular Sequence Data, Arabidopsis, Chromosome Mapping, Exons, RNA, Transfer, Amino Acyl, Genes, Plant, [SDV] Life Sciences [q-bio], Amino Acyl-tRNA Synthetases, Humans, Amino Acid Sequence
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