Increased Renal Responsiveness to Vasopressin and Enhanced V2 Receptor Signaling in RGS2 −/− Mice
Copyright policy )Increased Renal Responsiveness to Vasopressin and Enhanced V2 Receptor Signaling in RGS2 −/− Mice
The antidiuretic effect of vasopressin is mediated by V2 receptors (V2R) that are located in kidney connecting tubules and collecting ducts. This study provides evidence that V2R signaling is negatively regulated by regulator of G protein signaling 2 (RGS2), a member of the family of RGS proteins. This study demonstrates that (1) RGS2 expression in the kidney is restricted to the vasopressin-sensitive part of the nephron (thick ascending limb, connecting tubule, and collecting duct); (2) expression of RGS2 is rapidly upregulated by vasopressin; (3) the vasopressin-dependent accumulation of cAMP, the principal messenger of V2R signaling, is significantly higher in collecting ducts that are microdissected from the RGS2(-/-) mice compared with their wild-type littermates; and (4) analysis of urine output of mice that were exposed to water restriction followed by acute water loading revealed that RGS2(-/-) mice exhibit an increased renal responsiveness to vasopressin. It is proposed that RGS2 is involved in negative feedback regulation of V2R signaling.
- University of Lausanne Switzerland
- Institute of Molecular Biotechnology Austria
Feedback, Physiological, Male, Receptors, Vasopressin, Endothelin-1, Antidiuretic Agents, Sodium, Drinking, Nephrons, Mice, Mutant Strains, Cell Line, Mice, Inbred C57BL, Mice, Phenylephrine, Cyclic AMP, Animals, RNA, Messenger, Kidney Tubules, Collecting, Sympathomimetics, RGS Proteins, Signal Transduction
Feedback, Physiological, Male, Receptors, Vasopressin, Endothelin-1, Antidiuretic Agents, Sodium, Drinking, Nephrons, Mice, Mutant Strains, Cell Line, Mice, Inbred C57BL, Mice, Phenylephrine, Cyclic AMP, Animals, RNA, Messenger, Kidney Tubules, Collecting, Sympathomimetics, RGS Proteins, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).30 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!