BCR–ABL activity measured by 50% inhibitory concentration for imatinib, p-CrkL/CrkL ratio or p-CrkL ratio in CD34+ cells of patients with chronic myeloid leukemia does not predict treatment response
Copyright policy )BCR–ABL activity measured by 50% inhibitory concentration for imatinib, p-CrkL/CrkL ratio or p-CrkL ratio in CD34+ cells of patients with chronic myeloid leukemia does not predict treatment response
In this study, we assessed BCR-ABL kinase activity by measuring the protein levels of CrkL and its phosphorylated form (p-CrkL) in order to predict the clinical outcome of newly diagnosed chronic myeloid leukemia patients. CD34+ cells from these patients were collected before the start of imatinib therapy and treated in vitro with imatinib. The reduction of p-CrkL and CrkL protein levels was then measured by flow cytometry. The data were processed using three independent approaches: an assessment of a) IC50imatinib, b) p-CrkL/CrkL ratio, and c) p-CrkL ratio. The results were subsequently correlated with the clinical response of patients after 3 and 6 months of imatinib therapy. None of the three p-CrkL parameters measured in CD34+ cells was found to be predictive of clinical outcome.
- Masaryk University Czech Republic
- Central European Institute of Technology Czech Republic
Gene Expression Regulation, Leukemic, Fusion Proteins, bcr-abl, Nuclear Proteins, Antigens, CD34, Antineoplastic Agents, Flow Cytometry, Models, Biological, Piperazines, Inhibitory Concentration 50, Pyrimidines, Treatment Outcome, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Benzamides, Imatinib Mesylate, Humans, Adaptor Proteins, Signal Transducing, Signal Transduction
Gene Expression Regulation, Leukemic, Fusion Proteins, bcr-abl, Nuclear Proteins, Antigens, CD34, Antineoplastic Agents, Flow Cytometry, Models, Biological, Piperazines, Inhibitory Concentration 50, Pyrimidines, Treatment Outcome, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Benzamides, Imatinib Mesylate, Humans, Adaptor Proteins, Signal Transducing, Signal Transduction
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