AbstractNeuroD is a basic helix‐loop‐helix (bHLH) transcription factor critical for determining neuronal cell fate and regulating withdrawal from the cell cycle. We showed previously that, in goldfish,neuroDis expressed in the rod photoreceptor lineage, and we inferred thatneuroDis also expressed in a subset of amacrine cells and nascent cone photoreceptors. Here we extended that study by examining the temporal and spatial expression pattern ofneuroDin the embryonic and larval zebrafish and by identifying the cell types that express this gene.NeuroDexpression in the developing zebrafish retina is dynamic, spanning early retinogenesis and the maturation of cone photoreceptors. In early retinogenesisneuroDexpression expands from a small patch in the ventronasal retina, through the remaining retinal neuroepithelium. As retinogenesis progresses,neuroDexpression becomes restricted to amacrine cells, immature cones, and cells of rod and cone lineages. This expression achieves an adult pattern by 96 hours postfertilization (hpf), whereupon the temporal pattern ofneuroDexpression in central retina is spatially recapitulated at the germinative margin. The cellular pattern of expression suggests that neuroD regulates aspects of rod and cone genesis, but through separate cellular lineages. Furthermore,neuroDis coexpressed with the cone‐rod‐homeobox transcription factor (Crx) in putative cone progenitors and nascent cone photoreceptors, suggesting that, in the zebrafish retina, as in other vertebrate retinas, similar genetic cascades regulate photoreceptor genesis and maturation. J. Comp. Neurol. 501:1–12, 2007. © 2007 Wiley‐Liss, Inc.