Opposite Roles of the F-Box Protein Rcy1p and the GTPase-Activating Protein Gyp2p During Recycling of Internalized Proteins in Yeast
Copyright policy )Opposite Roles of the F-Box Protein Rcy1p and the GTPase-Activating Protein Gyp2p During Recycling of Internalized Proteins in Yeast
Abstract The F-box protein Rcy1p is part of a non-SCF (Skp1p-cullin-F-box protein) complex involved in recycling of internalized material. Like rcy1Δ, cells lacking the Rab-GTPase Ypt6p or its heterodimeric GEFs Rgp1p and Ric1p are unable to recycle the v-SNARE Snc1p. Here we provide genetic evidence suggesting that Rcy1p is a positive regulator of Ypt6p. Deletion of the GAP Gyp2p restores recycling in rcy1Δ, while overexpression of an active form of Ypt6p partially suppresses the recycling defect of rcy1Δ cells. Conversely, overexpression of Gyp2p in wild-type cells interferes with recycling of GFP-Snc1p, and the cells accumulate membrane structures as evidenced by electron microscopy. Gyp2p-GFP is distributed throughout the cytoplasm and accumulates in punctate structures, which concentrate in an actin-dependent manner at sites of polarized growth. Taken together, our results suggest that the F-box protein Rcy1p may activate the Ypt6p GTPase module during recycling.
- ETH Zurich Switzerland
- Institut Jacques Monod France
- University of Paris France
- Inserm France
- Institut des Sciences Biologiques France
Cytoplasm, Genotype, Models, Genetic, F-Box Proteins, Blotting, Western, GTPase-Activating Proteins, Green Fluorescent Proteins, Membrane Proteins, Biological Transport, DNA, Actins, Luminescent Proteins, Microscopy, Electron, Mutation, DNA Transposable Elements, Guanine Nucleotide Exchange Factors, Dimerization, Gene Deletion, Monomeric GTP-Binding Proteins, Plasmids
Cytoplasm, Genotype, Models, Genetic, F-Box Proteins, Blotting, Western, GTPase-Activating Proteins, Green Fluorescent Proteins, Membrane Proteins, Biological Transport, DNA, Actins, Luminescent Proteins, Microscopy, Electron, Mutation, DNA Transposable Elements, Guanine Nucleotide Exchange Factors, Dimerization, Gene Deletion, Monomeric GTP-Binding Proteins, Plasmids
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