Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation
Copyright policy )Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation
Assembly of functioning testis and ovary requires a GATA4-FOG2 transcriptional complex. To define the separate roles for GATA4 and FOG2 proteins in sexual development of the testis we have ablated the corresponding genes in somatic gonadal cells. We have established that GATA4 is required for testis differentiation, for the expression of Dmrt1 gene, and for testis cord morphogenesis. While Sf1Cre-mediated excision of Gata4 permitted normal expression of most genes associated with embryonic testis development, gonadal loss of Fog2 resulted in an early partial block in male pathway and sex reversal. We have also determined that testis sexual differentiation is sensitive to the timing of GATA4 loss during embryogenesis. Our results now demonstrate that these two genes also have non-overlapping essential functions in testis development.
- Harvard Stem Cell Institute, Cambridge, MA, USA United States
- Harvard University United States
- Harvard Medical School United States
- Dartmouth College United States
- Boston Children's Hospital United States
Male, Mice, 129 Strain, Sex Differentiation, GATA4, Mice, Testis, Animals, Genes, sry, Molecular Biology, Mice, Knockout, Dmrt1, Sertoli Cells, Ovary, Gene Expression Regulation, Developmental, SOX9 Transcription Factor, Cell Biology, Sex determination, FOG2, Mice, Mutant Strains, GATA4 Transcription Factor, DNA-Binding Proteins, Mice, Inbred C57BL, Female, Developmental Biology, Transcription Factors
Male, Mice, 129 Strain, Sex Differentiation, GATA4, Mice, Testis, Animals, Genes, sry, Molecular Biology, Mice, Knockout, Dmrt1, Sertoli Cells, Ovary, Gene Expression Regulation, Developmental, SOX9 Transcription Factor, Cell Biology, Sex determination, FOG2, Mice, Mutant Strains, GATA4 Transcription Factor, DNA-Binding Proteins, Mice, Inbred C57BL, Female, Developmental Biology, Transcription Factors
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