Heterozygous deletion of sarcolipin maintains normal cardiac function
Copyright policy )Heterozygous deletion of sarcolipin maintains normal cardiac function
Sarcolipin (SLN) is a small proteolipid and a regulator of sarco(endo)plasmic reticulum Ca2+-ATPase. In heart tissue, SLN is exclusively expressed in the atrium. Previously, we inserted Cre recombinase into the endogenous SLN locus by homologous recombination and succeeded in generating SLN-Cre knockin (SlnCre/+) mice. This SlnCre/+ mouse can be used to generate an atrium-specific gene-targeting mutant, and it is based on the Cre-loxP system. In the present study, we used adult SlnCre/+ mice atria and analyzed the effects of heterozygous SLN deletion by Cre knockin before use as the gene targeting mouse. Both SLN mRNA and protein levels were decreased in SlnCre/+ mouse atria, but there were no morphological, physiological, or molecular biological abnormalities. The properties of contractility and Ca2+ handling were similar to wild-type (WT) mice, and expression levels of several stress markers and sarcoplasmic reticulum-related protein levels were not different between SlnCre/+ and WT mice. Moreover, there was no significant difference in sarco(endo)plasmic reticulum Ca2+-ATPase activity between the two groups. We showed that SlnCre/+ mice were not significantly different from WT mice in all aspects that were examined. The present study provides basic characteristics of SlnCre/+ mice and possibly information on the usefulness of SlnCre/+ mice as an atrium-specific gene-targeting model.
- Kyoto University Japan
- Affiliated Hospital of Hangzhou Normal University China (People's Republic of)
- Institute of Science Tokyo Japan
- University of California, Los Angeles United States
- Hangzhou Normal University China (People's Republic of)
Male, Mice, Knockout, Heterozygote, Genotype, Proteolipids, Isoproterenol, Muscle Proteins, Adrenergic beta-Agonists, Fibrosis, Myocardial Contraction, Ventricular Function, Left, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Mice, Inbred C57BL, Sarcoplasmic Reticulum, Phenotype, Animals, Female, Myocytes, Cardiac, Calcium Signaling, Gene Deletion
Male, Mice, Knockout, Heterozygote, Genotype, Proteolipids, Isoproterenol, Muscle Proteins, Adrenergic beta-Agonists, Fibrosis, Myocardial Contraction, Ventricular Function, Left, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Mice, Inbred C57BL, Sarcoplasmic Reticulum, Phenotype, Animals, Female, Myocytes, Cardiac, Calcium Signaling, Gene Deletion
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