Identification, molecular cloning, and characterization of subunit 11 of the human 26S proteasome
Copyright policy )Identification, molecular cloning, and characterization of subunit 11 of the human 26S proteasome
We sequenced five peptides from subunit 11 (S11), a 43 kDa protein of the human 26S proteasome, and used this information to clone its cDNA. The S11 cDNA encodes a 376 amino acid protein with a pI of 5.6 and a molecular mass of 42.9 kDa. Translation of S11 RNA in the presence of [35S]methionine produces a radiolabeled protein that co‐migrates with S11 of the human 26S proteasome on SDS‐PAGE. Polyclonal antiserum made against recombinant S11 recognizes a protein of the same size in extracts of bacteria expressing S11 and in purified 26S proteasomes from human red blood cells or rabbit reticulocytes. The S11 sequence does not contain motifs that suggest a biological function. S11 is, however, the human homolog of Rpn9, a recently identified subunit of the yeast 26S proteasome.
- University of Utah United States
Adenosine Triphosphatases, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Ubiquitin, Molecular Sequence Data, Rpn9, 19S regulatory complex, S11, Human chromosome 11, Mice, Animals, Humans, Amino Acid Sequence, Rabbits, Cloning, Molecular, 26S proteasome
Adenosine Triphosphatases, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Ubiquitin, Molecular Sequence Data, Rpn9, 19S regulatory complex, S11, Human chromosome 11, Mice, Animals, Humans, Amino Acid Sequence, Rabbits, Cloning, Molecular, 26S proteasome
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).3 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!