Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
Copyright policy )Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein. hARH1 has been cloned, expressed heterologously in Escherichia coli, purified and crystallized in complex with K(+) and ADP. The orthorhombic crystals contained one monomer per asymmetric unit, exhibited a solvent content of 43% and diffracted X-rays to a resolution of 1.9 A. A prerequisite for obtaining well diffracting crystals was the performance of X-ray fluorescence analysis on poorly diffracting apo hARH1 crystals, which revealed the presence of trace amounts of K(+) in the crystal. Adding K-ADP to the crystallization cocktail then resulted in a crystal of different morphology and with dramatically improved diffraction properties.
- European Molecular Biology Laboratory Germany
- Institut für Immunologie Germany
- University Medical Center Hamburg-Eppendorf Germany
- European Bioinformatics Institute United Kingdom
X-Rays, Gene Expression, Cloning, Molecular, Crystallization, Crystallography, X-Ray, N-Glycosyl Hydrolases, Fluorescence, Enzymes
X-Rays, Gene Expression, Cloning, Molecular, Crystallization, Crystallography, X-Ray, N-Glycosyl Hydrolases, Fluorescence, Enzymes
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