Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH
Copyright policy )Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH
Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.
- Stanford University United States
- Memorial Sloan Kettering Cancer Center United States
TATA-Binding Protein Associated Factors, Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Genetic Vectors, Molecular Sequence Data, Gene Expression, Saccharomyces cerevisiae, Spodoptera, Polymerase Chain Reaction, Cyclin-Dependent Kinases, Protein Subunits, Cyclins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Animals, Humans, Electrophoresis, Polyacrylamide Gel, Transcription Factor TFIID, Amino Acid Sequence, Baculoviridae, Glutathione Transferase
TATA-Binding Protein Associated Factors, Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Genetic Vectors, Molecular Sequence Data, Gene Expression, Saccharomyces cerevisiae, Spodoptera, Polymerase Chain Reaction, Cyclin-Dependent Kinases, Protein Subunits, Cyclins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Animals, Humans, Electrophoresis, Polyacrylamide Gel, Transcription Factor TFIID, Amino Acid Sequence, Baculoviridae, Glutathione Transferase
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