Pax4 regulatory elements mediate beta cell specific expression in the pancreas
Copyright policy )Pax4 regulatory elements mediate beta cell specific expression in the pancreas
Pax4 is a member of the Pax (Pax, paired box) family of transcription factors with restricted expression and essential functions in the developing pancreas. Pax4 deficient mice do not develop pancreatic beta and delta cells and thus die after birth due to diabetes. In this study using transgenic mouse technology we report the identification and characterization of a 0.9 kb DNA fragment in the 5'-region of the gene, which by itself is able to direct Pax4 expression in the endocrine pancreas, recapitulating the beta-cell-specific in vivo expression pattern of Pax4. The 0.9 kb DNA fragment contains an area spanning 407 base pairs that is highly conserved between human and mouse showing 88% identity. This promoter region contains sequence motifs that have been shown to be involved in beta-cell-specific expression of insulin, Pdx1 and islet amyloid polypeptide (IAPP). In addition, we determined a previously undescribed 5'intron.
Homeodomain Proteins, Mice, Knockout, Embryology, Amyloid, Mice, Inbred BALB C, Models, Genetic, LIM-Homeodomain Proteins, Molecular Sequence Data, Nuclear Proteins, Mice, Transgenic, Nerve Tissue Proteins, Immunohistochemistry, Islet Amyloid Polypeptide, DNA-Binding Proteins, Islets of Langerhans, Mice, Hepatocyte Nuclear Factor 3-beta, Animals, Humans, Paired Box Transcription Factors, Amino Acid Sequence, Developmental Biology
Homeodomain Proteins, Mice, Knockout, Embryology, Amyloid, Mice, Inbred BALB C, Models, Genetic, LIM-Homeodomain Proteins, Molecular Sequence Data, Nuclear Proteins, Mice, Transgenic, Nerve Tissue Proteins, Immunohistochemistry, Islet Amyloid Polypeptide, DNA-Binding Proteins, Islets of Langerhans, Mice, Hepatocyte Nuclear Factor 3-beta, Animals, Humans, Paired Box Transcription Factors, Amino Acid Sequence, Developmental Biology
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