Two nuclear localization signals are required for nuclear translocation of nuclear factor 1‐A
Copyright policy )Two nuclear localization signals are required for nuclear translocation of nuclear factor 1‐A
Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1‐A, B, C, X). Although DNA‐binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1‐A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1‐A (NF1‐A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1‐A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.
- Osaka University Japan
- Nagoya City University Japan
Green Fluorescent Proteins, Molecular Sequence Data, Nuclear Localization Signals, Translocation, DNA-binding protein, Transfection, Nuclear localization signal, Nuclear factor 1, Humans, Amino Acid Sequence, Amino Acids, Cell Nucleus, Nuclear Proteins, Exons, DNA-Binding Proteins, Luminescent Proteins, NFI Transcription Factors, Protein Transport, CCAAT-Enhancer-Binding Proteins, Y-Box-Binding Protein 1, Transcription factor, Peptides, HeLa Cells, Transcription Factors
Green Fluorescent Proteins, Molecular Sequence Data, Nuclear Localization Signals, Translocation, DNA-binding protein, Transfection, Nuclear localization signal, Nuclear factor 1, Humans, Amino Acid Sequence, Amino Acids, Cell Nucleus, Nuclear Proteins, Exons, DNA-Binding Proteins, Luminescent Proteins, NFI Transcription Factors, Protein Transport, CCAAT-Enhancer-Binding Proteins, Y-Box-Binding Protein 1, Transcription factor, Peptides, HeLa Cells, Transcription Factors
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