Isolation and quantification of the heat shock protein 90 alpha and beta isoforms from rat liver
doi: 10.1007/bf01288360
pmid: 11732320
Isolation and quantification of the heat shock protein 90 alpha and beta isoforms from rat liver
Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and identified as the Hsp90 alpha and Hsp90 beta isoforms. There was no entry in the protein databases for the Hsp90 alpha isoform from rat. Furthermore, the ratio of the two Hsp90 isoforms was determined.
- Goethe University Frankfurt Germany
Male, Molecular Sequence Data, Rats, Molecular Weight, Rats, Sprague-Dawley, Liver, Animals, Humans, Protein Isoforms, Amino Acid Sequence, HSP90 Heat-Shock Proteins, Sequence Alignment
Male, Molecular Sequence Data, Rats, Molecular Weight, Rats, Sprague-Dawley, Liver, Animals, Humans, Protein Isoforms, Amino Acid Sequence, HSP90 Heat-Shock Proteins, Sequence Alignment
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