And the cell morphology was evaluated under the light microscope. B) Caspase-3 activity assay of rapamycin- and Ly294002-treated breast cancer cell lines. C) Caspase-3 activity assay of siRNA-treated breast cancer cell lines. The values are averages from two or three independent experiments. Ly294002 induced apoptosis in BT-474 and MDA-361 breast cancer cells, whereas rapamycin had no effect. Similarly, knock-down of did not have any effect on apoptosis in BT-474 and MCF-7 breast cancer cells.Copyright information:Taken from "Deciphering downstream gene targets of PI3K/mTOR/p70S6K pathway in breast cancer"http://www.biomedcentral.com/1471-2164/9/348BMC Genomics 2008;9():348-348.Published online 24 Jul 2008PMCID:PMC2496917.