Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot‐and‐mouth disease virus 3C protease: identification of the eIF4AI cleavage site
Copyright policy )Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot‐and‐mouth disease virus 3C protease: identification of the eIF4AI cleavage site
The translation initiation factor eIF4A is cleaved within mammalian cells infected by foot‐and‐mouth disease virus (FMDV). The FMDV 3C protease cleaves eIF4AI (between residues E143 and V144), but not the closely related eIF4AII. Modification of eIF4AI, to produce a sequence identical to eIF4AII around the cleavage site, blocked proteolysis. Alignment of mammalian eIF4AI onto the three‐dimensional structure of yeast eIF4A located the scissile bond within an exposed, flexible portion of the molecule. The N‐ and C‐terminal cleavage products of eIF4AI generated by FMDV 3C dissociate. Cleavage of eIF4AI by FMDV 3C is thus expected to inactivate it.
- University of Copenhagen Denmark
- University of Dundee United Kingdom
- Pirbright Institute United Kingdom
- Biotechnology and Biological Sciences Research Council United Kingdom
- University of Copenhagen Denmark
Models, Molecular, Protein Conformation, Molecular Sequence Data, Peptide Mapping, 3C protease, Substrate Specificity, Fungal Proteins, Viral Proteins, Peptide Initiation Factors, Translation initiation factor eIF4A, Animals, Humans, Amino Acid Sequence, Cells, Cultured, Binding Sites, Sequence Homology, Amino Acid, Foot-and-mouth disease virus, Picornavirus, 3C Viral Proteases, Recombinant Proteins, Cysteine Endopeptidases, Foot-and-Mouth Disease Virus, Eukaryotic Initiation Factor-4A, Protein synthesis
Models, Molecular, Protein Conformation, Molecular Sequence Data, Peptide Mapping, 3C protease, Substrate Specificity, Fungal Proteins, Viral Proteins, Peptide Initiation Factors, Translation initiation factor eIF4A, Animals, Humans, Amino Acid Sequence, Cells, Cultured, Binding Sites, Sequence Homology, Amino Acid, Foot-and-mouth disease virus, Picornavirus, 3C Viral Proteases, Recombinant Proteins, Cysteine Endopeptidases, Foot-and-Mouth Disease Virus, Eukaryotic Initiation Factor-4A, Protein synthesis
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