An Essential Role for RIG-I in Toll-like Receptor-Stimulated Phagocytosis
Copyright policy )An Essential Role for RIG-I in Toll-like Receptor-Stimulated Phagocytosis
Retinoic acid-inducible gene-I (RIG-I) plays an important role in antiviral response by recognizing double-stranded RNA. Here we demonstrate an unanticipated role of RIG-I in Toll-like receptor (TLR)-stimulated phagocytosis. Stimulation with lipopolysaccharide (LPS), a ligand of TLR4, induced the expression of RIG-I in macrophages. Depletion of RIG-I by RNAi or gene targeting inhibited the LPS-induced phagocytosis of bacteria. Cellular processes involved in phagocytosis, such as small GTPase Cdc42/Rac1 activation, actin polymerization, and actin-regulator Arp2/3 recruitment, were also impaired in RIG-I-deficient macrophages activated by LPS. Moreover, RIG-I(-/-) mice were found to be more susceptible to infection with Escherichia coli as compared to wild-type mice. Thus, the regulatory functions of RIG-I are strikingly broad, including a role not only in antiviral responses but in antibacterial responses as well.
- Chinese Academy of Sciences China (People's Republic of)
- Shanghai Institute of Nutrition and Health China (People's Republic of)
- Chinese Academy of Sciences, Institute of Zoology China (People's Republic of)
- Shanghai Institutes for Biological Sciences China (People's Republic of)
- State Key Laboratory of Medical Genomics China (People's Republic of)
rac1 GTP-Binding Protein, Cancer Research, MICROBIO, Actin-Related Protein 2-3 Complex, Cell Line, DEAD-box RNA Helicases, Mice, Phagocytosis, Immunology and Microbiology(all), Escherichia coli, Animals, Gene Silencing, RNA, Small Interfering, MOLIMMUNO, Molecular Biology, Escherichia coli Infections, Mice, Knockout, Macrophages, GTPase-Activating Proteins, Neuropeptides, Survival Analysis, rac GTP-Binding Proteins, Toll-Like Receptor 4, DEAD Box Protein 58, CELLBIO
rac1 GTP-Binding Protein, Cancer Research, MICROBIO, Actin-Related Protein 2-3 Complex, Cell Line, DEAD-box RNA Helicases, Mice, Phagocytosis, Immunology and Microbiology(all), Escherichia coli, Animals, Gene Silencing, RNA, Small Interfering, MOLIMMUNO, Molecular Biology, Escherichia coli Infections, Mice, Knockout, Macrophages, GTPase-Activating Proteins, Neuropeptides, Survival Analysis, rac GTP-Binding Proteins, Toll-Like Receptor 4, DEAD Box Protein 58, CELLBIO
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