Structure of the Small Ubiquitin-like Modifier (SUMO)-interacting Motif of MBD1-containing Chromatin-associated Factor 1 Bound to SUMO-3
Copyright policy )Structure of the Small Ubiquitin-like Modifier (SUMO)-interacting Motif of MBD1-containing Chromatin-associated Factor 1 Bound to SUMO-3
Post-translational modification by small ubiquitin-like modifier (SUMO) proteins has been implicated in the regulation of a variety of cellular events. The functions of sumoylation are often mediated by downstream effector proteins harboring SUMO-interacting motifs (SIMs) that are composed of a hydrophobic core and a stretch of acidic residues. MBD1-containing chromatin-associated factor 1 (MCAF1), a transcription repressor, interacts with SUMO-2/3 and SUMO-1, with a preference for SUMO-2/3. We used NMR spectroscopy to solve the solution structure of the SIM of MCAF1 bound to SUMO-3. The hydrophobic core of the SIM forms a parallel beta-sheet pairing with strand beta2 of SUMO-3, whereas its C-terminal acidic stretch seems to mediate electrostatic interactions with a surface area formed by basic residues of SUMO-3. The significance of these electrostatic interactions was shown by mutations of both SUMO-3 and MCAF1. The present structural and biochemical data suggest that the acidic stretch of the SIM of MCAF1 plays an important role in the binding to SUMO-3.
- Yokohama City University Japan
- Kyoto University Japan
- Institute of Protein Research Russian Federation
- RIKEN Japan
- Kumamoto University Japan
Amino Acid Motifs, Static Electricity, Repressor Proteins, Mutation, Humans, Protein Structure, Quaternary, Hydrophobic and Hydrophilic Interactions, Nuclear Magnetic Resonance, Biomolecular, Protein Processing, Post-Translational, Ubiquitins, Protein Binding, Transcription Factors
Amino Acid Motifs, Static Electricity, Repressor Proteins, Mutation, Humans, Protein Structure, Quaternary, Hydrophobic and Hydrophilic Interactions, Nuclear Magnetic Resonance, Biomolecular, Protein Processing, Post-Translational, Ubiquitins, Protein Binding, Transcription Factors
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