L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins
Copyright policy )L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins
L63 encodes a CDK-like protein homologous to the mammalian PFTAIRE. We showed previously that L63 provides a CDK-related function critical to development (Dev. Biol. 221 (2000) 23). We present here the first biochemical characterization of L63 kinase. In addition, we describe two novel Drosophila proteins, PIF-1 and PIF-2 (for PFTAIRE Interacting Factor-1 and -2), identified in a two-hybrid screen for their ability to interact with the amino-terminal region of L63. The full-length PIF-1 cDNA shows an unusual dicistronic organization. PIF-1A and PIF-1B (the L63 interactor) predicted proteins are expressed in vivo, and show a distinct expression profile during development. Interaction between L63 and PIF-1B was confirmed in vitro and in vivo. The role of this interaction remains to be demonstrated, but our data suggest that PIF-1B might serve as a regulator of L63.
- Florida Southern College United States
- University of Paris France
- University of Paris France
- Stanford University United States
- Institut Jacques Monod France
Embryology, DNA, Complementary, Models, Genetic, Blotting, Western, Molecular Sequence Data, Gene Expression Regulation, Developmental, Exons, Blotting, Northern, Precipitin Tests, Cyclin-Dependent Kinases, Protein Structure, Tertiary, Protein Biosynthesis, Animals, Drosophila Proteins, Humans, Protein Isoforms, Drosophila, Amino Acid Sequence, Cysteine, Cloning, Molecular, Developmental Biology, Protein Binding
Embryology, DNA, Complementary, Models, Genetic, Blotting, Western, Molecular Sequence Data, Gene Expression Regulation, Developmental, Exons, Blotting, Northern, Precipitin Tests, Cyclin-Dependent Kinases, Protein Structure, Tertiary, Protein Biosynthesis, Animals, Drosophila Proteins, Humans, Protein Isoforms, Drosophila, Amino Acid Sequence, Cysteine, Cloning, Molecular, Developmental Biology, Protein Binding
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