Saccharomyces cerevisiae RNA Polymerase I Terminates Transcription at the Reb1 Terminator In Vivo
Copyright policy )Saccharomyces cerevisiae RNA Polymerase I Terminates Transcription at the Reb1 Terminator In Vivo
We have mapped transcription termination sites for RNA polymerase I in the yeast Saccharomyces cerevisiae. S1 nuclease mapping shows that the primary terminator is the Reb1p terminator located at +93 downstream of the 3' end of 25S rRNA. Reverse transcription coupled with quantitative PCR shows that approximately 90% of all transcripts terminate at this site. Transcripts which read through the +93 site quantitatively terminate at a fail-safe terminator located further downstream at +250. Inactivation of Rnt1p (an RNase III involved in processing the 3' end of 25S rRNA) greatly stabilizes transcripts extending to both sites and increases readthrough at the +93 site. In vivo assay of mutants of the Reb1p terminator shows that this site operates in vivo by the same mechanism as has previously been delineated through in vitro studies.
- Central University of Venezuela Venezuela
- Fred Hutchinson Cancer Research Center South Africa
- Fred Hutchinson Cancer Research Center United States
Ribonuclease III, Terminator Regions, Genetic, Saccharomyces cerevisiae Proteins, Base Sequence, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, DNA-Binding Proteins, RNA Polymerase I, RNA, Ribosomal, Endoribonucleases, Mutation, RNA Processing, Post-Transcriptional, DNA Probes, Protein Binding, Transcription Factors
Ribonuclease III, Terminator Regions, Genetic, Saccharomyces cerevisiae Proteins, Base Sequence, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, DNA-Binding Proteins, RNA Polymerase I, RNA, Ribosomal, Endoribonucleases, Mutation, RNA Processing, Post-Transcriptional, DNA Probes, Protein Binding, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).36 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!