A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG‐1/hSNF2β and localizes in the Golgi apparatus
Copyright policy )A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG‐1/hSNF2β and localizes in the Golgi apparatus
An ATP‐dependent chromatin remodeling factor, SNF/SWI complex, acts as a coactivator for numerous transcriptional factors. One of the best‐documented examples is nuclear receptors, although the molecular mechanism for this coactivation has not been sufficiently elucidated. Here we show that hbrm/hSNF2α and BRG‐1/hSNF2β, the ATPase subunits of the human SNF/SWI complexes, specifically associate in vitro and in vivo with TATA element modulatory factor (TMF)/ARA160, which has been described as a binding protein to and coactivator for the androgen receptor. This interaction requires highly conserved N‐terminal regions of hbrm/hSNF2α and BRG‐1/hSNF2β and a C‐terminal region of TMF/ARA160. Immunofluorescence and Western blot studies revealed that the TMF isoforms differentially localize in the Golgi apparatus and the nucleus.
- Hokkaido Bunkyo University Japan
- Hokkeido University Japan
- Hokkaido University Japan
Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Blotting, Western, Green Fluorescent Proteins, Golgi Apparatus, Saccharomyces cerevisiae, Tumor Cells, Cultured, Animals, Humans, Adenosine Triphosphatases, Binding Sites, Cofactor, DNA Helicases, Nuclear Proteins, SWI/SNF, DNA-Binding Proteins, Luminescent Proteins, Microscopy, Fluorescence, Nuclear receptor, Golgi apparatus, COS Cells, Transcription, HeLa Cells, Protein Binding, Transcription Factors
Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Blotting, Western, Green Fluorescent Proteins, Golgi Apparatus, Saccharomyces cerevisiae, Tumor Cells, Cultured, Animals, Humans, Adenosine Triphosphatases, Binding Sites, Cofactor, DNA Helicases, Nuclear Proteins, SWI/SNF, DNA-Binding Proteins, Luminescent Proteins, Microscopy, Fluorescence, Nuclear receptor, Golgi apparatus, COS Cells, Transcription, HeLa Cells, Protein Binding, Transcription Factors
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