Boric acid reversibly inhibits the second step of pre‐mRNA splicing
Copyright policy )Boric acid reversibly inhibits the second step of pre‐mRNA splicing
Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre‐mRNA splicing reaction causes a dose‐dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice‐site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre‐mRNA splicing.
- Tel Aviv University Israel
RNA Splicing, In Vitro Techniques, Boric acid, Ribonucleoproteins, Small Nuclear, Boronic Acids, Recombinant Proteins, Globins, Kinetics, Pre-mRNA splicing, Spliceosome, Boric Acids, Metals, RNA Precursors, Spliceosomes, Humans, RNA Splicing Factors, Inhibition
RNA Splicing, In Vitro Techniques, Boric acid, Ribonucleoproteins, Small Nuclear, Boronic Acids, Recombinant Proteins, Globins, Kinetics, Pre-mRNA splicing, Spliceosome, Boric Acids, Metals, RNA Precursors, Spliceosomes, Humans, RNA Splicing Factors, Inhibition
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