Type 1 Inositol 1,4,5-Trisphosphate Receptor Is Required for Induction of Long-Term Depression in Cerebellar Purkinje Neurons
Copyright policy )Type 1 Inositol 1,4,5-Trisphosphate Receptor Is Required for Induction of Long-Term Depression in Cerebellar Purkinje Neurons
The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+channel that releases Ca2+from internal Ca2+stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+influx through Ca2+channels on the plasma membrane, Ca2+release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.
- University of Tokyo Japan
- RIKEN Japan
Neuronal Plasticity, Patch-Clamp Techniques, Antibodies, Monoclonal, Receptors, Cytoplasmic and Nuclear, Inositol 1,4,5-Trisphosphate, In Vitro Techniques, Mice, Mice, Neurologic Mutants, Purkinje Cells, Antibody Specificity, Animals, Inositol 1,4,5-Trisphosphate Receptors, Calcium Channels
Neuronal Plasticity, Patch-Clamp Techniques, Antibodies, Monoclonal, Receptors, Cytoplasmic and Nuclear, Inositol 1,4,5-Trisphosphate, In Vitro Techniques, Mice, Mice, Neurologic Mutants, Purkinje Cells, Antibody Specificity, Animals, Inositol 1,4,5-Trisphosphate Receptors, Calcium Channels
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).210 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!