Supplement Figure 5. LNP-mediated over-expression of FAM13A increases FAM13A protein expression in 1HAEo cells. A) Assessment of FAM13A gene expression relative to GAPDH in 1HAEo cells treated with empty LNP or LNP-FAM13A mRNA in the absence or presence of TGFβ1 treatment. B) Quantification of FAM13A protein expression normalized to β-actin in 1HAEo cells treated with empty LNP or LNP-FAM13A mRNA in the absence or presence of TGFβ1 treatment. Values were expressed as mean ± SEM (N=3 independent experiments). One-way analysis of variance with Bonferroni’s multiple comparisons test was used in all panels.