Additional file 1: Figure S1. (A) The expression of circSERPINA3 was detected via RT-qPCR after WPMY-1 cells were transfected with pcDNA3.1-circSERPINA3. (B) WPMY-1 cell apoptosis was detected with the help of an analysis of annexin V staining by flow cytometry after circSERPINA3 overexpression. (C) Caspase3 Activity Assay Kit was applied to examine Caspase3 activity when circSERPINA3 was up-regulated in WPMY-1 cells. (D) After circSERPINA3 elevation, Caspase9 activity in WPMY-1 cells was detected by Caspase9 Activity Assay Kit. (E–F) The expression of apoptosis-associated proteins (Bcl-2, Bax and Cleaved-PARP1) was analyzed by western blot in PCa cells with sh-circSERPINA3-1/2 transfection. (G-H) Western blot was implemented to examine the expression of apoptosis-associated proteins in PCa cells transfected with pcDNA3.1-circSERPINA3. (I) The levels of cell autophagy-related proteins (LC3-I, LC3-II and p62) was detected via western blot in WPMY-1 cells with circSERPINA3 overexpression. (J-K) Cell autophagy was evaluated by means of western blot in PCa cells under different conditions (Rapamycin treatment or circSERPINA3 reduction). (L) Glucose consumption in WPMY-1 cells was tested after circSERPINA3 overexpression. (M) Lactate content was detected in WPMY-1 cells transfected with pcDNA3.1-circSERPINA3. (N) PK activity was examined in WPMY-1 cells with circSERPINA3 up-regulation. (O) ECAR/OCR value in WPMY-1 cells was tested under the condition of circSERPINA3 elevation. (sh: short hairpin; NC: negative control). **P < 0.01.