Influence of a deletion of protein kinase Cγ isoform in the G-protein activation mediated through opioid receptor-like-1 and μ-opioid receptors in the mouse pons/medulla
Copyright policy )Influence of a deletion of protein kinase Cγ isoform in the G-protein activation mediated through opioid receptor-like-1 and μ-opioid receptors in the mouse pons/medulla
The aim of the present study was to investigate whether mice with a deletion of the gene that encodes the protein kinase C gamma (PKC gamma) isoform could affect the G-protein activation mediated through the opioid receptor-like (ORL-1) receptor and mu-opioid receptor in the mouse pons/medulla and spinal cord, monitoring the guanosine-5'-o-(3-[(35)S]thio) triphosphate ([(35)S]GTP gamma S) binding assay. The increases in [(35)S]GTP gamma S bindings to pons/medulla membranes of the wild-type mice induced by either an endogenous ligand for the ORL-1 receptor, nociceptin or a selective mu-opioid receptor agonist [D-Ala(2),N-MePhe(4),Gly-ol(5)]enkephalin were significantly enhanced in PKC gamma knockout mice. In contrast, the levels of [(35)S]GTP gamma S binding stimulated by nociceptin in spinal cord membranes obtained from PKC gamma knockout mice were similar to those from wild-type mice. These findings suggest that the loss of the PKC gamma gene may protect the functional ORL-1 and mu-opioid receptors from degradation by phosphorylation in the mouse pons/medulla. Furthermore, the present data provide first evidence for the differential mechanism of the ORL-1 receptor-mediated signaling between the supraspinal and spinal sites.
- Medical College of Wisconsin United States
- Hoshi University Japan
Mice, Knockout, Medulla Oblongata, Nociceptin, Receptors, Opioid, mu, Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, Nociceptin Receptor, Isoenzymes, Mice, Opioid Peptides, Spinal Cord, GTP-Binding Proteins, Guanosine 5'-O-(3-Thiotriphosphate), Pons, Receptors, Opioid, Animals, Gene Deletion, Protein Kinase C
Mice, Knockout, Medulla Oblongata, Nociceptin, Receptors, Opioid, mu, Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, Nociceptin Receptor, Isoenzymes, Mice, Opioid Peptides, Spinal Cord, GTP-Binding Proteins, Guanosine 5'-O-(3-Thiotriphosphate), Pons, Receptors, Opioid, Animals, Gene Deletion, Protein Kinase C
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).7 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!