Loss of STAG2 causes aneuploidy in normal human bladder cells
Copyright policy )Loss of STAG2 causes aneuploidy in normal human bladder cells
The aim of this study was to determine how the function of human stromal antigen 2 (STAG2) plays an important role in proper chromosome separation. STAG2 mRNA in normal bladder cells and bladder tumor cells was evaluated by RT-PCR. The protein levels of STAG2 in normal bladder cells and bladder tumor cells were determined by western blot. A cell proliferation assay was used to measure the growth of tumor cells and STAG2-inhibited normal cells, and STAG2- inhibited normal cells were subjected to karyotype analysis. Both STAG-2 mRNA and protein expression levels were lower in bladder cancer cells compared to the controls. Knockdown of STAG2 caused aneuploidy in normal bladder cells, leading to a decreased expression of the cohesin complex components SMC1, SMC3 and RAD21, but there was no obvious effect of STAG2 knockdown on cell proliferation. Our study indicated that abnormal expression of STAG2 could cause aneuploidy in normal bladder cells.
- Peking University China (People's Republic of)
- Hong Kong University of Science and Technology (香港科技大學) China (People's Republic of)
- Shenzhen University China (People's Republic of)
- Peking University Shenzhen Hospital China (People's Republic of)
- Third Affiliated Hospital of Southern Medical University China (People's Republic of)
Chromosomal Proteins, Non-Histone, Blotting, Western, Urinary Bladder, Gene Expression, Cell Cycle Proteins, Cell Line, Cell Line, Tumor, Stromal antigen 2, Humans, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Bladder cancer, Nuclear Proteins, Antigens, Nuclear, Aneuploidy, Phosphoproteins, Urologic neoplasms, DNA-Binding Proteins, Chondroitin Sulfate Proteoglycans, Urinary Bladder Neoplasms, Karyotyping, RNA Interference, Cohesin complex, Structural Maintenance of Chromosome Protein 1
Chromosomal Proteins, Non-Histone, Blotting, Western, Urinary Bladder, Gene Expression, Cell Cycle Proteins, Cell Line, Cell Line, Tumor, Stromal antigen 2, Humans, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Bladder cancer, Nuclear Proteins, Antigens, Nuclear, Aneuploidy, Phosphoproteins, Urologic neoplasms, DNA-Binding Proteins, Chondroitin Sulfate Proteoglycans, Urinary Bladder Neoplasms, Karyotyping, RNA Interference, Cohesin complex, Structural Maintenance of Chromosome Protein 1
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