High temperature promotes an improved activity of the Saccharomyces cerevisiae glycerol/H+ symporter encoded by STL1, which correlates well with Stl1p levels. This happens in both fermentable and respiratory metabolic growth conditions, though the induction in the latter is much higher. The relief of glucose repression by high temperature at the level of protein expression and activity (Stl1p) is reported for the first time. We reason that the glycerol internal levels fine‐tuning, under heat‐stress as in other physiological condition, can be achieved with the contribution of the tight regulation of the symporter.