Function of COP9 Signalosome in Regulation of Mouse Oocytes Meiosis by Regulating MPF Activity and Securing Degradation
Copyright policy )Function of COP9 Signalosome in Regulation of Mouse Oocytes Meiosis by Regulating MPF Activity and Securing Degradation
The COP9 (constitutive photomorphogenic) signalosome (CSN), composed of eight subunits, is a highly conserved protein complex that regulates processes such as cell cycle progression and kinase signalling. Previously, we found the expression of the COP9 constitutive photomorphogenic homolog subunit 3 (CSN3) and subunit 5 (CSN5) changes as oocytes mature for the first time, and there is no report regarding roles of COP9 in the mammalian oocytes. Therefore, in the present study, we examined the effects of RNA interference (RNAi)-mediated transient knockdown of each subunit on the meiotic cell cycle in mice oocytes. Following knockdown of either CSN3 or CSN5, oocytes failed to complete meiosis I. These arrested oocytes exhibited a disrupted meiotic spindle and misarranged chromosomes. Moreover, down-regulation of each subunit disrupted the activity of maturation-promoting factor (MPF) and concurrently reduced degradation of the anaphase-promoting complex/cyclosome (APC/C) substrates Cyclin B1 and Securin. Our data suggest that the CSN3 and CSN5 are involved in oocyte meiosis by regulating degradation of Cyclin B1 and Securin via APC/C.
- Stanford University United States
- CHA University Korea (Republic of)
- CHA Advanced Research Institute Korea (Republic of)
Science, Maturation-Promoting Factor, Down-Regulation, Fluorescent Antibody Technique, Models, Biological, Mice, Animals, Cyclin B1, Mice, Inbred ICR, COP9 Signalosome Complex, Q, R, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Cell Differentiation, Cell Cycle Checkpoints, Chromosomes, Mammalian, Meiosis, Gene Knockdown Techniques, Mesothelin, Multiprotein Complexes, Medicine, Female, Carrier Proteins, Research Article
Science, Maturation-Promoting Factor, Down-Regulation, Fluorescent Antibody Technique, Models, Biological, Mice, Animals, Cyclin B1, Mice, Inbred ICR, COP9 Signalosome Complex, Q, R, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Cell Differentiation, Cell Cycle Checkpoints, Chromosomes, Mammalian, Meiosis, Gene Knockdown Techniques, Mesothelin, Multiprotein Complexes, Medicine, Female, Carrier Proteins, Research Article
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