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Nature Methods
Article . 2011 . Peer-reviewed
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Nature Methods
Article . 2011
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Nature Methods
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Other literature type . 2011
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A genome-scale shRNA resource for transgenic RNAi in Drosophila

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 03 Apr 2011 United States, Canada English Publisher:Springer Science and Business Media LLCJournal:Nature Methods, volume 8, pages 405-407 (issn: 1548-7091, eissn: 1548-7105, Copyright policy )Funded by:NIH | Functional Genomic Screen..., NIH | Drosophila Transgenic RNA..., EC | DROSOPIRNAS
Authors: Phillip Karpowicz; Lizabeth A. Perkins; Gregory J. Hannon; Hye Seok Shim; Norbert Perrimon; Julius Brennecke; Matthew A. Booker; +11 Authors

doi: 10.1038/nmeth.1592

pmid: 21460824

pmc: PMC3489273

A genome-scale shRNA resource for transgenic RNAi in Drosophila

Abstract

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

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Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics, 570, Base Sequence, Genetic Vectors, Genome, Insect, Life Sciences, Animals, Genetically Modified, MicroRNAs, Drosophila melanogaster, Oogenesis, Genetic Techniques, Gene Knockdown Techniques, Animals, Female, RNA Interference, RNA, Small Interfering, Biology, DNA Primers

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    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 731
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 0.1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 0.1%
Powered by OpenAIRE graph
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
731
Top 0.1%
Top 1%
Top 0.1%
bronze