Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies
Copyright policy )Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies
To understand the biological functions of the ectoplasmic specializations between Sertoli cells and maturing spermatids in seminiferous epithelia.In order to disrupt the function of the ectoplasmic specializations, nectin-2, which is expressed at the specialization, was neutralized with anti-nectin-2 antibody micro-injected into the lumen of the mouse seminiferous tubule. Anti-nectin-3 antibody was also micro-injected into the lumen in order to neutralize nectin-3, which is expressed at the specialization.The actin filaments at the specialization disappeared, and exfoliation of maturing spermatids was observed by electron microscopy.Nectin-2 was neutralized by anti-nectin-2 antibody and nectin-3 was neutralized by anti-nectin-3 antibody, respectively. Inactivated nectin-2 and nectin-3 disrupted the nectin-afadin-actin system, and finally the actin filaments disappeared. As a result, the specialization lost the holding function and detachment of spermatids was observed. One of the functions of the specialization seems to be to hold maturing spermatids until spermiation.
- Chiba University Japan
Male, Mice, Inbred ICR, Microscopy, Confocal, Sertoli Cells, Microfilament Proteins, Nectins, Cell Communication, Spermatids, Actins, Antibodies, Mice, Intercellular Junctions, Seminiferous Epithelium, Animals, Cell Adhesion Molecules
Male, Mice, Inbred ICR, Microscopy, Confocal, Sertoli Cells, Microfilament Proteins, Nectins, Cell Communication, Spermatids, Actins, Antibodies, Mice, Intercellular Junctions, Seminiferous Epithelium, Animals, Cell Adhesion Molecules
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).10 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!