Cloning and functional characterization of the human sodium‐dependent vitamin C transporters hSVCT1 and hSVCT2
Copyright policy )Cloning and functional characterization of the human sodium‐dependent vitamin C transporters hSVCT1 and hSVCT2
Two sodium‐dependent vitamin C transporters, hSVCT1 and hSVCT2, were cloned from a human kidney cDNA library. hSVCT1 had a 1797 bp open reading frame encoding a 598 amino acid polypeptide. The 1953 bp open reading frame of hSVCT2 encoded a 650 amino acid polypeptide. Using a Xenopus laevis oocyte expression system, both transporters were functionally expressed. By Eadie‐Hofstee transformation the apparent K m of hSVCT1 for ascorbate was 252.0 μM and of hSVCT2 for ascorbate was 21.3 μM. Both transporters were sodium‐dependent and did not transport dehydroascorbic acid. Incubation of oocytes expressing either transporter with phorbol 12‐myristate 13‐acetate (PMA) inhibited ascorbate transport activity. Availability of the human transporter clones may facilitate new strategies for determining vitamin C intake.
- National Institute of Health Pakistan
- National Institute of Diabetes and Digestive and Kidney Diseases United States
- National Institutes of Health United States
- National Institute of Health (NIH/NICHD) United States
Molecular Sequence Data, Transport, Organic Anion Transporters, Sodium-Dependent, Ascorbic Acid, Xenopus laevis, Animals, Humans, Ascorbate, Vitamin C, Amino Acid Sequence, Cloning, Molecular, Sodium-Coupled Vitamin C Transporters, Base Sequence, Symporters, Sodium, Proteins, Biological Transport, Bucladesine, Protein Biosynthesis, hSVCT2, hSVCT1, Oocytes, Tetradecanoylphorbol Acetate, Female, Carrier Proteins
Molecular Sequence Data, Transport, Organic Anion Transporters, Sodium-Dependent, Ascorbic Acid, Xenopus laevis, Animals, Humans, Ascorbate, Vitamin C, Amino Acid Sequence, Cloning, Molecular, Sodium-Coupled Vitamin C Transporters, Base Sequence, Symporters, Sodium, Proteins, Biological Transport, Bucladesine, Protein Biosynthesis, hSVCT2, hSVCT1, Oocytes, Tetradecanoylphorbol Acetate, Female, Carrier Proteins
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).254 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!