ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations
Copyright policy )ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations
A variety of stimuli can trigger intracellular calcium oscillations. Relatively little is known about the molecular mechanisms decoding these events. We show that ALG-2, a Ca2+-binding protein originally isolated as a protein associated with apoptosis, is directly linked to Ca2+ signalling. We discovered that the subcellular distribution of a tagged version of ALG-2 could be directed by physiological external stimuli (including ATP, EGF, prostaglandin, histamine), which provoke intracellular Ca2+ oscillations. Cellular stimulation led to a redistribution of ALG-2 from the cytosol to a punctate localization in an oscillatory fashion unitemporally with Ca2+ oscillations, whereas a Ca2+-binding deficient mutant of ALG-2 did not redistribute. Using tagged ALG-2 as bait we identified its novel target protein Sec31A and based on the partial colocalization of endogenous ALG-2 and Sec31A we propose that ALG-2 temporarily binds to the COPII vesicles providing a link between Ca2+ signalling and ER to Golgi trafficking.
- University of Copenhagen Denmark
- University of Copenhagen Denmark
Cytoplasm, Microscopy, Confocal, Recombinant Fusion Proteins, Calcium-Binding Proteins, Green Fluorescent Proteins, Immunohistochemistry, Protein Transport, Adenosine Triphosphate, Cell Line, Tumor, Mutation, Humans, Immunoprecipitation, Calcium, Electrophoresis, Polyacrylamide Gel, Calcium Signaling, Apoptosis Regulatory Proteins, Carrier Proteins, HeLa Cells, Histamine, Protein Binding
Cytoplasm, Microscopy, Confocal, Recombinant Fusion Proteins, Calcium-Binding Proteins, Green Fluorescent Proteins, Immunohistochemistry, Protein Transport, Adenosine Triphosphate, Cell Line, Tumor, Mutation, Humans, Immunoprecipitation, Calcium, Electrophoresis, Polyacrylamide Gel, Calcium Signaling, Apoptosis Regulatory Proteins, Carrier Proteins, HeLa Cells, Histamine, Protein Binding
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