The 19S proteasomal lid subunit POH1 enhances the transcriptional activation by Mitf in osteoclasts
Copyright policy )The 19S proteasomal lid subunit POH1 enhances the transcriptional activation by Mitf in osteoclasts
AbstractThe microphthalmia‐associated transcription factor (Mitf) regulates gene expression required for osteoclast differentiation. Genes regulated by Mitf have been previously identified. However, proteins that interact and regulate Mitf's activity in osteoclasts are not well known. Here, we report that POH1, a subunit of the 19S proteasome lid is a regulator of Mitf. We show that POH1 and Mitf interact in osteoclasts and that this interaction is dependent on RANKL signaling. Overexpression of POH1 increased Mitf's activation of 5XGal4‐TK and Acp5 promoters. The amino terminus of POH1 mediates the binding to Mitf and is sufficient to increase Mitf's transcriptional activity. Finally, we show that mutations in the JAMM motif of POH1 reduced Mitf activation of promoters. In summary, our results identify a novel mechanism of Mitf regulation in osteoclasts by POH1. J. Cell. Biochem. 109: 967–974, 2010. © 2010 Wiley‐Liss, Inc.
- University of Minnesota Morris United States
- University of Minnesota System United States
- University of Minnesota United States
Transcriptional Activation, Microphthalmia-Associated Transcription Factor, Proteasome Endopeptidase Complex, Macrophages, Amino Acid Motifs, Molecular Sequence Data, Osteoclasts, Cell Differentiation, Protein Structure, Tertiary, Jurkat Cells, Mice, Protein Subunits, Two-Hybrid System Techniques, NIH 3T3 Cells, Trans-Activators, Animals, Humans, Amino Acid Sequence, Protein Processing, Post-Translational, Protein Binding
Transcriptional Activation, Microphthalmia-Associated Transcription Factor, Proteasome Endopeptidase Complex, Macrophages, Amino Acid Motifs, Molecular Sequence Data, Osteoclasts, Cell Differentiation, Protein Structure, Tertiary, Jurkat Cells, Mice, Protein Subunits, Two-Hybrid System Techniques, NIH 3T3 Cells, Trans-Activators, Animals, Humans, Amino Acid Sequence, Protein Processing, Post-Translational, Protein Binding
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