Novel Genes Required for Meiotic Chromosome Segregation Are Identified by a High-Throughput Knockout Screen in Fission Yeast
Copyright policy )Novel Genes Required for Meiotic Chromosome Segregation Are Identified by a High-Throughput Knockout Screen in Fission Yeast
Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination.
- University of Bucharest Romania
- University of Bern Switzerland
- Research Institute of Molecular Pathology Austria
Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Chromosomal Proteins, Non-Histone, Gene Expression Profiling, Genetic Vectors, Green Fluorescent Proteins, Forkhead Transcription Factors, Chromatids, Polymerase Chain Reaction, Meiosis, Microscopy, Fluorescence, Chromosome Segregation, Schizosaccharomyces, Streptothricins, Schizosaccharomyces pombe Proteins, Hygromycin B, Gene Deletion, DNA Primers
Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Chromosomal Proteins, Non-Histone, Gene Expression Profiling, Genetic Vectors, Green Fluorescent Proteins, Forkhead Transcription Factors, Chromatids, Polymerase Chain Reaction, Meiosis, Microscopy, Fluorescence, Chromosome Segregation, Schizosaccharomyces, Streptothricins, Schizosaccharomyces pombe Proteins, Hygromycin B, Gene Deletion, DNA Primers
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