Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
Copyright policy )Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology with the human connective tissue-activating peptide III (CTAP III). The human tumor cell cDNA hybridizing with the Chinese hamster clone was isolated, sequenced, and found to have closer similarity to the Chinese hamster gene than to CTAP III. Thus, the cloned cDNAs from Chinese hamster and human cells represent a different gene, named gro. Studies of its transcriptional regulation have shown that expression is tightly regulated by growth status in normal Chinese hamster and human cells and relaxed in the tumorigenic cells so far examined.
- Harvard University United States
- National Cancer Institute United States
- Dana-Farber Cancer Institute United States
- Division of Cancer Epidemiology and Genetics United States
Base Sequence, Chemokine CXCL1, Molecular Sequence Data, Nucleic Acid Hybridization, DNA, DNA, Neoplasm, Fibroblasts, Cell Transformation, Neoplastic, Cricetulus, Gene Expression Regulation, Cricetinae, Multigene Family, Animals, Humans, Intercellular Signaling Peptides and Proteins, Amino Acid Sequence, Peptides, Poly A, Chemokines, CXC, Cell Division
Base Sequence, Chemokine CXCL1, Molecular Sequence Data, Nucleic Acid Hybridization, DNA, DNA, Neoplasm, Fibroblasts, Cell Transformation, Neoplastic, Cricetulus, Gene Expression Regulation, Cricetinae, Multigene Family, Animals, Humans, Intercellular Signaling Peptides and Proteins, Amino Acid Sequence, Peptides, Poly A, Chemokines, CXC, Cell Division
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).336 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!