Figure S4: Analysis of knockdown efficiency in HIV-1-infected U2OS-C5 cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

Name: Figure S4: Analysis of knockdown efficiency in HIV-1-infected U2OS-C5 cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication
Keywords: 3. Good health

descriptionPublicationkeyboard_double_arrow_right Other literature type 01 Jan 2017Publisher:The Royal SocietyFunded by:DFG | unidentified

Authors: Hofmann, Stephan; Dehn, Sandra; Businger, Ramona; Bolduan, Sebastian; Schneider, Martha; Debyser, Zeger; Brack-Werner, Ruth; +1 Authors

doi: 10.6084/m9.figshare.5436589.v1 , 10.6084/m9.figshare.5436589

Figure S4: Analysis of knockdown efficiency in HIV-1-infected U2OS-C5 cells from Dual role of the chromatin-binding factor PHF13 in the pre- and post-integration phases of HIV-1 replication

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Abstract

U2OS-C5 cells were mock transfected or with a control siRNA (csiRNA) or directed against PHF13 (siPHF13). 24 h later cells were infected with 100 ng p24 VSVG pseudotyped HIV-1 NL4-3 IRES-eGFP or the ΔVpr variant for 6 h, washed and incubated for additional 18 h. At that time point, cells were induced with 1 μg/ml doxycycline. 24 h later cells were lysed to detect PHF13, tubulin, HIV-1 p24 and Vpr by immunoblot.

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