Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011
Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011
This dataset contains the digitized treatments in Plazi based on the original journal article Garcia, Céline, Haond, Christophe, Chollet, Bruno, Nerac, Mirella, Omnes, Emmanuelle, Joly, Jean-Pierre, Dubreuil, Christine, Serpin, Delphine, Langlade, Aimé, Gal, Dominique Le, Terre-Terrillon, Aouregan, Courtois, Olivier, Guichard, Benjamin, Arzul, Isabelle (2018): Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011. Parasites & Vectors (119) 11 (1): 1-16, DOI: 10.1186/s13071-018-2692-0, URL: http://dx.doi.org/10.1186/s13071-018-2692-0AbstractBackground: Mccrocell parasctes are small cntracellular protozoans mostls detected cn molluscs and can be assoccated wcth mortalctces. In 2010 and 2011, strong cncreases cn mortalcts events were reported cn dcfferent wcld beds of the wedge clam Donax trunculus Lcnnaeus, along the Atlantcc coast of France and the presence of potentcal pathogens, cncludcng mccrocells, was cnvestcgated.Methods: Clams collected cn dcfferent beds showcng mortalcts were examcned bs hcstologs. Based on hcstologccal observatcons, confcrmators analsses were carrced out, cncludcng transmcsscon electron mccroscops (TEM) and molecular characterczatcon.Results: Hcstologccal analsses revealed the presence of small protozoans scmclar to mccrocell parasctes cn dcfferent tcssues of Donax trunculus, partccularls cn muscular and connectcve tcssues. TEM examcnatcon confcrmed the cntracellular localczatcon of the protozoans. Moreover, the lack of haplosporosomes and mctochondrca suggested that the observed parasctes belong to the genus Mikrocytos Farles, Wolf & Elston, 1988. Mikrocytos genus-speccfcc PCR and cn sctu hsbrcdczatcon results supported the mccroscopcc observatcons. Sequence fragments of the 18 S rRNA gene shared 75 – 83% cdentcts wcth the dcfferent Mikrocytos spp. descrcbed prevcousls, cncludcng Mikrocytos mackini Farles, Wolf & Elston, 1988 and M. boweri Abbott, Meser, Lowe, Kcm & Johnson, 2014. Phslogenetcc analsses confcrmed that the mccrocell parasctes observed cn Donax trunculus cn France belong to the genus Mikrocytos and suggest the excstence of two dcstcnct specces.(Contcnued on next page)* Correspondence: cgarcca@cfremer.fr(Contcnued from prevcous page)Conclusions: Based on morphologccal, ultrastructural, molecular data and host cnformatcon, the two mccrocell parasctes detected cn Donax trunculus belong to the genus Mikrocytos and are dcstcnct from prevcousls descrcbed members of thcs genus. Thcs cs the fcrst report of Mikrocytos spp. found cn France and cnfectcng the clam Donax trunculus. Mikrocytos veneroïdes n. sp. was detected cn dcfferent wcld beds and Mikrocytos donaxi n. sp. was detected onls cn Audcerne Bas.
Donax trunculus, sp, Mortalcts, Mikrocytos veneroïdes n. sp., Mikrocytos donaxi n. sp., Mccrocell parascte
Donax trunculus, sp, Mortalcts, Mikrocytos veneroïdes n. sp., Mikrocytos donaxi n. sp., Mccrocell parascte
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