MiR-206 Regulates Neural Cells Proliferation and Apoptosis viaOtx2
Copyright policy )MiR-206 Regulates Neural Cells Proliferation and Apoptosis viaOtx2
MiR-206 was involved in a series of cellular activities, such as the growth and development of skeletal muscle and the tumorigenesis. MiR-206 was characterized previously as a differentially expressed gene in sodium arsenite (SA)-induced neural tube defects (NTDs) in chick embryos via miRNA microarray analysis. However, the role of miR-206 in the pathological process of nerve cells remained elusive. In this study we found differential expression of miR-206 in SA-treated chick embryos by Northern blot analysis. Ectopic expression of miR-206 inhibited cell proliferation, and promoted cell apoptosis in U343 and SK-N-SH cell by using MTT, Edu Apollo assay and Flow cytometry analysis. Further investigation revealed that miR-206 can interact with 3'-untranslated region (UTR) of Otx2. MiR-206 mimics down-regulated the endogeneous Otx2 expression, whereas the miR-206 inhibitor obviously up-regulated the expression of Otx2. These findings indicate that overexpression of miR-206 promotes cell apoptosis and low expression of miR-206 inhibits cell apoptosis. Otx2 may play an important role in the process of miR-206-mediated cell apoptosis.
- Chinese Academy of Medical Sciences & Peking Union Medical College China (People's Republic of)
Neurons, Binding Sites, Otx Transcription Factors, Arsenites, Cell Survival, Fluorescent Antibody Technique, Apoptosis, Chick Embryo, Glioma, Blotting, Northern, Flow Cytometry, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell Line, Tumor, Animals, Humans, Neural Tube Defects, 3' Untranslated Regions, Cell Proliferation, Plasmids
Neurons, Binding Sites, Otx Transcription Factors, Arsenites, Cell Survival, Fluorescent Antibody Technique, Apoptosis, Chick Embryo, Glioma, Blotting, Northern, Flow Cytometry, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell Line, Tumor, Animals, Humans, Neural Tube Defects, 3' Untranslated Regions, Cell Proliferation, Plasmids
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