A genetic in vivo system to detect asymmetrically distributed RNA
Copyright policy )A genetic in vivo system to detect asymmetrically distributed RNA
Many RNAs show polarized or otherwise non‐random subcellular distributions. To create a method for genome‐wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo .
- European Molecular Biology Laboratory Germany
- University of Cologne Germany
- European Bioinformatics Institute United Kingdom
- University of Washington United States
- College of New Jersey United States
Cell Nucleus, Genetic Vectors, Green Fluorescent Proteins, Reproducibility of Results, Pilot Projects, RNA Transport, Drosophila melanogaster, Organ Specificity, Protein Biosynthesis, Animals, RNA, Genetic Testing, Levivirus
Cell Nucleus, Genetic Vectors, Green Fluorescent Proteins, Reproducibility of Results, Pilot Projects, RNA Transport, Drosophila melanogaster, Organ Specificity, Protein Biosynthesis, Animals, RNA, Genetic Testing, Levivirus
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