Role of vascular endothelial growth factor and placental growth factor in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic mice
Copyright policy )Role of vascular endothelial growth factor and placental growth factor in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic mice
To evaluate the role of vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) in adhesion formation after laparoscopic surgery.Prospective, randomized study.Academic research center.Female wild-type mice and transgenic mice (n = 110), expressing exclusively VEGF-A(164) (VEGF-A(164/164)) or deficient for VEGF-B (VEGF-B(-/-)) or for PlGF (PlGF(-/-)).Adhesions were induced during laparoscopy. To evaluate "basal adhesions" and "CO(2) pneumoperitoneum-enhanced adhesions," the pneumoperitoneum was maintained for a minimum (10 minutes) or prolonged (60 minutes) period. The role of PlGF was also evaluated by administration of antibodies.Adhesions were blindly scored after 7 days.In all wild-type mice, CO(2) pneumoperitoneum enhanced adhesion formation. In comparison with wild-type mice, basal adhesions were higher in VEGF-A(164/164) mice and similar in VEGF-B(-/-) and PlGF(-/-) mice. Pneumoperitoneum did not enhance adhesions in any of these transgenic mice. The effects observed in PlGF(-/-) mice were confirmed in PlGF antibody-treated mice.The data demonstrate that the VEGF family plays a role in adhesion formation and confirm that CO(2) pneumoperitoneum enhances adhesions. VEGF-A(164) has a direct role in basal adhesions. Absence of pneumoperitoneum-enhanced adhesions in VEGF-A(164/164), VEGF-B(-/-), and PlGF(-/-) mice indicates up-regulation of VEGF-A(164), VEGF-B, and PlGF by CO(2) pneumoperitoneum as a mechanism for pneumoperitoneum-enhanced adhesion formation.
- Instituts Universitaires de Technologie France
- KU Leuven Belgium
- Ludwig Cancer Research Belgium
- Katholieke Universiteit Leuven Belgium
- Ludwig Cancer Research Sweden
Vascular Endothelial Growth Factor A, Lymphokines, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, Tissue Adhesions, Endothelial Growth Factors, Carbon Dioxide, Pregnancy Proteins, Mice, Inbred C57BL, Mice, Random Allocation, Postoperative Complications, Animals, Intercellular Signaling Peptides and Proteins, Female, Laparoscopy, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial, Placenta Growth Factor
Vascular Endothelial Growth Factor A, Lymphokines, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, Tissue Adhesions, Endothelial Growth Factors, Carbon Dioxide, Pregnancy Proteins, Mice, Inbred C57BL, Mice, Random Allocation, Postoperative Complications, Animals, Intercellular Signaling Peptides and Proteins, Female, Laparoscopy, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial, Placenta Growth Factor
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