Expression of mouse telomerase catalytic subunit in embryos and adult tissues
Copyright policy )Expression of mouse telomerase catalytic subunit in embryos and adult tissues
Telomerase is a ribonucleoprotein complex that elongates telomeres, allowing the stable maintenance of chromosomes during multiple cell divisions. Here, we describe the isolation and characterization of the catalytic subunit of mouse telomerase, mTERT ( m ouse te lomerase r everse t ranscriptase), an essential protein component of the telomerase complex. During embryonic development, mTERT mRNA is abundantly expressed in the whole embryo, especially in regions of intense proliferation. We found that the mTERT mRNA expression in both embryonic and adult tissues is independent of the essential RNA component of telomerase, mTR, and therefore, of the formation of active telomerase complexes. mTERT protein is present exclusively in tissues with telomerase activity, such as testis, spleen, and thymus. mTERT protein is barely detectable in the thymus of mTR −/− mice, suggesting that mTERT protein stability in this tissue may depend on the actual assembly of active telomerase complexes. Finally, we found that mouse and human telomerase catalytic subunit is located in the cell nucleus, and its localization is not regulated during cell cycle progression.
Base Sequence, Molecular Sequence Data, Gene Expression Regulation, Developmental, Proteins, Embryo, Mammalian, Antibodies, Catalysis, Gene Expression Regulation, Enzymologic, DNA-Binding Proteins, Mice, Animals, Humans, RNA, Cloning, Molecular, Telomerase, DNA Primers
Base Sequence, Molecular Sequence Data, Gene Expression Regulation, Developmental, Proteins, Embryo, Mammalian, Antibodies, Catalysis, Gene Expression Regulation, Enzymologic, DNA-Binding Proteins, Mice, Animals, Humans, RNA, Cloning, Molecular, Telomerase, DNA Primers
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